荧光
化学
微流控
微珠(研究)
磁性纳米粒子
纳米颗粒
纳米技术
微流控芯片
量子点
磁选
聚合物
材料科学
生物化学
有机化学
物理
冶金
量子力学
作者
Xiaoqun Gong,Hongwei Yan,Jiumin Yang,Yudong Wu,Jian Zhang,Yingyi Yao,Ping Liu,Huiquan Wang,Zhidong Hu,Jin Chang
标识
DOI:10.1016/j.aca.2016.08.031
摘要
Fluorescence-encoded magnetic microbeads (FEMMs), with the fluorescence encoding ability of quantum dots (QDs) and magnetic enrichment and separation functions of Fe3O4 nanoparticles, have been widely used for multiple biomolecular detection as microfluidic protein chip supports. However, the preparation of FEMMs with long-term fluorescent encoding and immunodetection stability is still a challenge. In this work, we designed a novel high-temperature chemical swelling strategy. The QDs and Fe3O4 nanoparticles were effectively packaged into microbeads via the thermal motion of the polymer chains and the hydrophobic interaction between the nanoparticles and microbeads. The FEMMs obtained a highly uniform fluorescent property and long-term encoding and immunodetection stability and could be quickly magnetically separated and enriched. Then, the QD-encoded magnetic microbeads were applied to alpha fetoprotein (AFP) detection via sandwich immunoreaction. The properties of the encoded microspheres were characterized using a self-designed detecting apparatus, and the target molecular concentration in the sample was also quantified. The results suggested that the high-performance FEMMs have great potential in the field of biomolecular detection.
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