From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

甘露聚糖 酵母 甘露糖苷酶 酿酒酵母 乙醇燃料 生物化学 发酵 甘露糖 化学 乙醇发酵 多糖 食品科学
作者
Jun Ishii,Fumiyoshi Okazaki,Apridah Cameliawati Djohan,Kiyotaka Y. Hara,Nanami Asai-Nakashima,Hiroshi Teramura,Ade Andriani,Masahiro Tominaga,Satoshi Wakai,Prihardi Kahar,- Yopi,Bambang Prasetya,Prihardi Kahar,Akihiko Kondo
出处
期刊:Biotechnology for Biofuels [Springer Science+Business Media]
卷期号:9 (1) 被引量:29
标识
DOI:10.1186/s13068-016-0600-4
摘要

Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced in sustainable biofuel development. Based on a yeast cell surface-display technology that enables the immobilization of multiple enzymes on the yeast cell walls, we constructed a recombinant Saccharomyces cerevisiae strain that co-displays β-mannanase and β-mannosidase; this strain is expected to facilitate ethanol fermentation using mannan as a biomass source.Parental yeast S. cerevisiae assimilated mannose and glucose as monomeric sugars, producing ethanol from mannose. We constructed yeast strains that express tethered β-mannanase and β-mannosidase; co-display of the two enzymes on the cell surface was confirmed by immunofluorescence staining and enzyme activity assays. The constructed yeast cells successfully hydrolyzed 1,4-β-d-mannan and produced ethanol by assimilating the resulting mannose without external addition of enzymes. Furthermore, the constructed strain produced ethanol from 1,4-β-d-mannan continually during the third batch of repeated fermentation. Additionally, the constructed strain produced ethanol from ivory nut mannan; ethanol yield was improved by NaOH pretreatment of the substrate.We successfully displayed β-mannanase and β-mannosidase on the yeast cell surface. Our results clearly demonstrate the utility of the strain co-displaying β-mannanase and β-mannosidase for ethanol fermentation from mannan biomass. Thus, co-tethering β-mannanase and β-mannosidase on the yeast cell surface provides a powerful platform technology for yeast fermentation toward the production of bioethanol and other biochemicals from lignocellulosic materials containing mannan components.
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