检出限
过氧化氢
线性范围
辣根过氧化物酶
过氧化物酶
化学
分析化学(期刊)
色谱法
材料科学
生物化学
酶
作者
Aida Alaei,Morteza Hosseini,Ehsan Shokri,Mohammad Reza Ganjali
标识
DOI:10.1002/slct.202104389
摘要
Abstract Abnormal glucose level can cause irreversible damage, including diabetes. In this work, a sensor was designed that mimics the peroxidase property of the natural horseradish peroxidase (HRP). The proposed sensor detects the presence of glucose through the reduction of hydrogen peroxide (H 2 O 2 ) and the oxidation of tetramethylbenzidine (TMB). This sensor shows linear behaviour in the range of 0.001–50 μM with a limit of detection down to 0.8 nM. In addition, a paper‐based format of this sensing platform was performed which enables fast and sensitive detection and further reduces the expenses. In this method the linear behaviour of the sensor is between 0.005–40 μM and the detection limit is as low as 2.4 nM. The glucose detection in human saliva was also performed, showing an acceptable accuracy. Thus the sensor is practically suitable for the non‐invasive detection of glucose.
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