严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
环介导等温扩增
病毒学
清脆的
痰
荧光
2019年冠状病毒病(COVID-19)
纳米技术
化学
材料科学
生物
物理
医学
基因
DNA
传染病(医学专业)
光学
病理
肺结核
生物化学
疾病
作者
Qin Zhang,Jiahao Li,Yue Li,Guangyun Tan,Minmin Sun,Yanke Shan,Yue Zhang,Xin Wang,Keyu Song,Rui Shi,Ling Huang,Fei Liu,Yongxiang Yi,Xuping Wu
标识
DOI:10.1016/j.bios.2022.113978
摘要
The development of reliable, sensitive, and fast devices for the diagnosis of COVID-19 is of great importance in the pandemic of the new coronavirus. Here, we proposed a new principle of analysis based on a combination of reverse transcription and isothermal amplification of a fragment of the gene encoding the S protein of the SARS-CoV-2 and the CRISPR/Cas13a reaction for cleavage of the specific probe. As a result, the destroyed probe cannot be detected on an immunochromatographic strip using quantum fluorescent dots. Besides, the results can be obtained by an available and inexpensive portable device. By detecting SARS-CoV-2 negative (n = 25) and positive (n = 62) clinical samples including throat swabs, sputum and anal swabs, the assay showed good sensitivity and specificity of the method and could be completed within 1 h without complicated operation and expensive equipment. These superiorities showed its potential for fast point-of-care screening of SARS-CoV-2 during the outbreak, especially in remote and underdeveloped areas with limited equipment and resources.
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