褐藻糖胶
生物分子
纳米颗粒
伤口愈合
壳聚糖
化学
纳米技术
材料科学
生物物理学
多糖
医学
生物化学
免疫学
生物
作者
Kandasamy Saravanakumar,Zijun Li,Yebon Kim,Seon Ju Park,Keon Kim,Chang-Min Lee,Ginnae Ahn,Namki Cho
标识
DOI:10.1016/j.envres.2023.118004
摘要
The colonization of pathogenic microbes is a major clinical barrier that suppresses the physiological wound-healing process. Therefore, the development of a novel wound dressing to combat bacterial infection is essential for treatment of infected wounds. Herein, we developed a modified cotton gauze dressing coated with fucoidan and functionalized with silver nanoparticles (LB-Ag NPs–FN–OCG) for the rapid treatment of infected wounds. Firstly, the phytocompound-capped LB-Ag NPs were synthesized and characterized by high performance liquid chromatography (HPLC), transmission electron microscopy (TEM), and zeta potential analysis. Secondly, the different concentrations of LB-Ag NPs (0.1%–1%) were functionalized into FN-OCG to screen appropriate concentrations that were non-toxic with superior antibacterial activities. The screening assays, which included an antibacterial, hemolysis, Chick Chorioallantoic Membrane (CAM) assay, and cytotoxicity assay, revealed that LB-Ag NPs (0.5%) –FN–OCG were found to be non-toxic and had a greater efficiency in inhibiting the bacteria pathogens (E. coli, S. enterica, S. aureus, and L. monocytogenes) and promoting fibroblast cell (NIH3T3) migration. In vivo assays revealed that LB-Ag NPs (0.5%)–FN–OCG treatment exhibited excellent wound healing activity (99.73 ± 0.01%) compared to other treatments by inhibiting bacterial colonization and maintaining the blood parameters, developing granulation tissue, new blood vessels, and collagen deposition. Overall, this study revealed that LB-Ag NPs (0.5%)–FN–OCG can act as a non-toxic, antibacterial wound dressing for infected wound healing applications.
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