再生医学
生物相容性
纳米纤维素
生物医学工程
间充质干细胞
材料科学
脚手架
再生(生物学)
纳米技术
骨愈合
3D生物打印
化学
组织工程
外科
细胞
纤维素
细胞生物学
医学
有机化学
生物
生物化学
冶金
作者
Markel Lafuente-Merchan,Sandra Ruiz-Alonso,Fátima García‐Villén,Alaitz Zabala,Ana M. Ochoa de Retana,Idoia Gallego,Laura Saenz‐del‐Burgo,José Luis Pedraz
标识
DOI:10.1002/mabi.202200236
摘要
Bone tissue is usually damaged after big traumas, tumors, and increasing aging-related diseases such as osteoporosis and osteoarthritis. Current treatments are based on implanting grafts, which are shown to have several inconveniences. In this regard, tissue engineering through the 3D bioprinting technique has arisen to manufacture structures that would be a feasible therapeutic option for bone regenerative medicine. In this study, nanocellulose-alginate (NC-Alg)-based bioink is improved by adding two different inorganic components such as hydroxyapatite (HAP) and graphene oxide (GO). First, ink rheological properties and biocompatibility are evaluated as well as the influence of the sterilization process on them. Then, scaffolds are characterized. Finally, biological studies of embedded murine D1 mesenchymal stem cells engineered to secrete erythropoietin are performed. Results show that the addition of both HAP and GO prevents NC-Alg ink from viscosity lost in the sterilization process. However, GO is reduced due to short cycle autoclave sterilization, making it incompatible with this ink. In addition, HAP and GO have different influences on scaffold architecture and surface as well as in swelling capacity. Scaffolds mechanics, as well as cell viability and functionality, are promoted by both elements addition. Additionally, GO demonstrates an enhanced bone differentiation capacity.
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