Hypothermic Effects of Neuropeptide FF Analogues in Mice

The effects of neuropeptide FF (NPFF) and its analogues on mouse body temperature were examined. In a thermoneutral environment, administration of NPFF (Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-NH2), 1DMe ([D.Tyr1, (N.Me)Phe3] NPFF), and 3D ([D.Tyr1, D.Leu2, D.Phe3] NPFF) in the third ventricle produced marked hypothermia. The effect of 1DMe was dose-dependent, and 45 nmol decreased body temperature by 5.6 degrees C. This effect was more pronounced when mice were placed at 4 degrees C. Hypothermia was not reversed by naloxone, an opioid antagonist, and was not modified by morphine. After 5 days of chronic treatment with 1DMe, mice did not became tolerant to the hypothermic effect. These results indicate that central NPFF receptors may control body temperature independently from opioid functions.