Progress on Protein / Peptide Phosphorylation

Phosphorylation is one of the most important post-translational modifications of proteins, which is related to many activities of life.By reversible protein phosphorylation eukaryotes control many cellular processes including signal transduction, gene expression, and the cell cycle etc.As the development and application of the proteomics, the studies of the protein phosphorylation have become more important.This article has introduced the main types and functions of the protein phosphorylation, the enrichment and preparation of phosphoproteins and phosphopeptides, the identification of the phosphopeptides, the determination and prediction of the specific-phosphorylation-site, the phosphorelated modifications of the proteins, and the progress on studies above as well.