光合反应器
蓝藻
光合作用
环境科学
生物燃料
生物能源
烟气
角鲨烯
制浆造纸工业
生化工程
化学
生物
植物
生物技术
废物管理
工程类
细菌
生物化学
遗传学
作者
Sun Young Choi,Sang Jun Sim,Sung Cheon Ko,Jigyeong Son,Jeong Seop Lee,Hyun Jeong Lee,Won Seok Chang,Han Min Woo
标识
DOI:10.1021/acs.jafc.0c03133
摘要
Economically feasible photosynthetic cultivation of microalgal and cyanobacterial strains is crucial for the biological conversion of CO2 and potential CO2 mitigation to challenge global warming. To overcome the economic barriers, the production of value-added chemicals was desired by compensating for the overall processing cost. Here, we engineered cyanobacteria for photosynthetic squalene production and cultivated them in a scalable photobioreactor using industrial flue gas. First, an inducer-free gene expression system was developed for the cyanobacteria to lower production const. Then, the recombinant cyanobacteria were cultivated in a closed photobioreactor (100 L) using flue gas (5% CO2) as the sole carbon source under natural sunlight as the only energy source. Seasonal light intensities and temperatures were analyzed along with cyanobacterial cell growth and squalene production in August and October 2019. As a result, the effective irradiation hours were the most critical factor for the large-scale cultivation of cyanobacteria. Thus, an automated photobioprocess system will be developed based on the regional light sources.
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