糖基化
糖基转移酶
尿苷二磷酸
生物化学
拟南芥
山茶
生物
生物合成
生长素
基因
核苷酸糖
化学
拟南芥
非生物胁迫
次生代谢
酶
植物
突变体
作者
Chang-Li Yang,Fangyuan Tian,Jun Ma,Mei Chen,Xingxing Shi,Dingli Chen,Yurong Xie,Xingrong Zhou,Zhi Zhou,Xinlong Dai,Tao Xia,Liping Gao
标识
DOI:10.1021/acs.jafc.3c05843
摘要
Camellia sinensis contains numerous glycosylated secondary metabolites that provide various benefits to plants and humans. However, the genes that catalyze the glycosylation of multitype metabolites in tea plants remain unclear. Here, 180 uridine diphosphate-dependent glycosyltransferases that may be involved in the biosynthesis of glycosylated secondary metabolites were identified from the National Center for Biotechnology Information public databases. Subsequently, CsUGT74Y1 was screened through phylogenetic analysis and gene expression profiling. Compositional and induced expression analyses revealed that CsUGT74Y1 was highly expressed in tea tender shoots and was induced under biotic and abiotic stress conditions. In vitro enzymatic assays revealed that rCsUGT74Y1 encoded a multifunctional UGT that catalyzed the glycosylation of flavonoids, phenolic acids, lignins, and auxins. Furthermore, CsUGT74Y1-overexpressing Arabidopsis thaliana exhibited enhanced growth and accumulation of flavonol and auxin glucosides. Our findings provide insights into identifying specific UGTs and demonstrate that CsUGT74Y1 is a multifunctional UGT that promotes plant development.
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