多胺氧化酶
谷氨酸脱羧酶
平菇
谷氨酸脱氢酶
生物
新陈代谢
菌丝体
氧化酶试验
精氨酸脱羧酶
多胺
GABA转氨酶
γ-氨基丁酸
谷氨酸受体
细胞生物学
精胺
酶
生物化学
植物
受体
蘑菇
作者
Qing Wen,Haoyang Zhao,Yanhong Shao,Jiatao Li,Yanru Hu,Yuancheng Qi,Fengqin Wang,Jinwen Shen
标识
DOI:10.1016/j.foodres.2023.112549
摘要
GABA is a health-promoting bioactive substance. Here, the GABA biosynthetic pathways were investigated, and then the dynamic quantitative changes in GABA and the expression levels of genes related to GABA metabolism under heat stress or at different developmental stages of fruiting bodies in Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm were determined. We found that the polyamine degradation pathway was the main route of GABA production under growth normal condition. The accumulation of GABA and the expression of most genes related to GABA biosynthesis, including genes encoding glutamate decarboxylase (PoGAD-2), polyamine oxidase (PoPAO-1), diamine oxidase (PoDAO) and aminoaldehyde dehydrogenase (PoAMADH-1 and PoAMADH-2), were significantly suppressed by heat stress and the excessive maturity of fruiting bodies. Finally, the effects of GABA on the mycelial growth, heat tolerance and the morphogenesis and development of fruiting bodies were studied, the results showed that the deficiency of endogenous GABA inhibited the mycelial growth and primordial formation and aggravated heat damage, whereas exogenous application of GABA could improve thermotolerance and promote the development of fruiting bodies.
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