Reproducibility of NIRS-derived mitochondrial oxidative capacity in highly active older adults

In-vivo techniques using near-infrared spectroscopy (NIRS) have been developed to assess skeletal muscle mitochondrial oxidative capacity. However, the test-retest and day-to-day reliability of NIRS-derived mitochondrial oxidative capacity has yet to be established in older individuals. Therefore, the primary aim of this study was to determine the day-to-day and test-retest reliability of NIRS-derived mitochondrial oxidative capacity in older adults. The secondary aim was to examine the relationship between NIRS-derived mitochondrial capacity and whole-body aerobic fitness.Twenty-four healthy individuals (19 M, 5F; aged 60 ± 4 years; maximal oxygen uptake (V̇O2peak) = 41.2 ± 6.8 ml.kg-1.min-1) completed three visits to the laboratory. Visit one assessed isometric maximal voluntary contractions of the knee extensors and aerobic capacity through an incremental exercise test. In visits two and three participants completed two measurements of NIRS-derived mitochondrial oxidative capacity in the vastus lateralis (VL).NIRS-derived mitochondrial oxidative capacity was found to have good to excellent day-to-day reliability (Day 1 vs Day 2; coefficient of variation (CV) = 7.0 %; standard error of measurement (SEM) = 5.2; intra-class correlation coefficient (ICC) = 0.94) and test re-test reliability (Day 1 [Test 1 vs Test 2]; CV = 5.0 %; SEM = 3.7; ICC 0.97 and Day 2 [Test 1 vs Test 2]; CV = 6.3 %; SEM = 4.9; ICC = 0.93). NIRS-derived mitochondrial oxidative capacity was found to be significantly correlated with V̇O2peak (r = -0.61; R2 = 0.37; P = 0.002), oxygen uptake at the gas exchange threshold (r = -0.49; R2 = 0.24; P = 0.02), and oxygen uptake at the respiratory compensation point (r = -0.57; R2 = 0.32; P = 0.004).NIRS provides a reliable method for deriving a measure of VL mitochondrial oxidative capacity in highly active older adults and demonstrates a significant relationship with measures of whole-body aerobic fitness.