生物
MyoD公司
干细胞
细胞生物学
骨骼肌
心肌细胞
细胞外基质
眼外肌
转录因子
间充质干细胞
肌动蛋白
细胞分化
免疫学
解剖
基因
遗传学
肌发生
作者
Daniela Di Girolamo,Maria Benavente-Diaz,Melania Murolo,Alexandre Grimaldi,Priscilla Thomas Lopes,Brendan Evano,Mao Kiruki,Stamatia Gioftsidi,Vincent Laville,Jean‐Yves Tinevez,Gaëlle Letort,Sébastien Mella,Shahragim Tajbakhsh,Glenda Comai
出处
期刊:Development
[The Company of Biologists]
日期:2024-01-17
摘要
Skeletal muscle stem cells (MuSC) are recognized as functionally heterogeneous. Cranial MuSCs are reported to have greater proliferative and regenerative capacity when compared to the ones in the limb. A comprehensive understanding of the mechanisms underlying this functional heterogeneity is lacking. Here we used clonal analysis, live imaging and scRNA-seq to identify critical features that distinguish extraocular (EOM) from limb muscle stem cell populations. A MyogenintdTom reporter showed that the increased proliferation capacity of EOM MuSCs correlates with deferred differentiation and lower expression of the myogenic commitment gene Myod. Unexpectedly, in vitro activated EOM MuSCs expressed a large array of extracellular matrix components typical of mesenchymal non-muscle cells. Computational analysis underscored a distinct co-regulatory module, which is absent in limb MuSCs, as driver of these features. The EOM transcription factor network, with Foxc1 as key player, appears to be hardwired to EOM identity as it persists during growth, disease, and in vitro after several passages. Our findings shed light on how high-performing MuSCs regulate myogenic commitment by remodeling of their local environment and adopting properties not generally associated with myogenic cells.
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