西斯特
核糖核酸
染色质
X-失活
抄写(语言学)
长非编码RNA
遗传学
RNA沉默
生物
细胞生物学
计算生物学
X染色体
基因
RNA干扰
语言学
哲学
作者
Lisa Rodermund,Heather Coker,Roel Oldenkamp,Guifeng Wei,Joseph S Bowness,Bramman Rajkumar,Tatyana B. Nesterova,David Miguel Susano Pinto,Lothar Schermelleh,Neil Brockdorff
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2021-06-11
卷期号:372 (6547)
被引量:43
标识
DOI:10.1126/science.abe7500
摘要
X-inactive specific transcript (Xist) RNA directs the process of X chromosome inactivation in mammals by spreading in cis along the chromosome from which it is transcribed and recruiting chromatin modifiers to silence gene transcription. To elucidate mechanisms of Xist RNA cis-confinement, we established a sequential dual-color labeling, super-resolution imaging approach to trace individual Xist RNA molecules over time, which enabled us to define fundamental parameters of spreading. We demonstrate a feedback mechanism linking Xist RNA synthesis and degradation and an unexpected physical coupling between preceding and newly synthesized Xist RNA molecules. Additionally, we find that the protein SPEN, a key factor for Xist-mediated gene silencing, has a distinct function in Xist RNA localization, stability, and coupling behaviors. Our results provide insights toward understanding the distinct dynamic properties of Xist RNA.
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