核糖核酸
底漆延伸
核酶
底漆(化妆品)
RNA编辑
连接酶核酶
核苷酸
非编码RNA
RNA依赖性RNA聚合酶
小核RNA
内含子
生物
核酸酶保护试验
遗传学
化学
基因
有机化学
作者
Wendy K. Johnston,Peter J. Unrau,Michael S. Lawrence,Margaret E. Glasner,David P. Bartel
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2001-05-18
卷期号:292 (5520): 1319-1325
被引量:691
标识
DOI:10.1126/science.1060786
摘要
The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA replication. In support of this conjecture, we describe here an RNA molecule that catalyzes the type of polymerization needed for RNA replication. The ribozyme uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides-more than a complete turn of an RNA helix. Its polymerization activity is general in terms of the sequence and the length of the primer and template RNAs, provided that the 3' terminus of the primer pairs with the template. Its polymerization is also quite accurate: when primers extended by 11 nucleotides were cloned and sequenced, 1088 of 1100 sequenced nucleotides matched the template.
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