外周血单个核细胞
内皮
医学
病理
单核细胞浸润
T细胞
过继性细胞移植
免疫学
促炎细胞因子
移植
生物
炎症
免疫系统
体外
内科学
生物化学
作者
Denis A. Tereb,Nancy C. Kirkiles-Smith,Richard W. Kim,Yinong Wang,R. Daniel Rudic,Jeffrey S. Schechner,Marc I. Lorber,Alfred L.M. Bothwell,Jordan S. Pober,George Tellides
出处
期刊:Transplantation
[Ovid Technologies (Wolters Kluwer)]
日期:2001-06-01
卷期号:71 (11): 1622-1630
被引量:22
标识
DOI:10.1097/00007890-200106150-00023
摘要
We have previously demonstrated that human artery grafts transplanted to immunodeficient mice are infiltrated and injured by unsensitized allogeneic human T cells. We extended our investigations to human anti-porcine xenoresponses in this model.Pig coronary artery segments were interposed into the infrarenal aorta of severe combined immunodeficiency/beige mice. After 7 days, certain recipients were reconstituted with human leukocytes and/or treated with proinflammatory cytokines. The grafts were harvested after 1-70 days and examined by histology, immunohistochemistry, and morphometry.Pig artery grafts from untreated mice had no evidence of injury, leukocytic infiltrate, or endothelial cell activation up to 70 days postoperatively, despite deposition of murine complement. Host reconstitution with human peripheral blood mononuclear cells resulted in a discrete population of circulating T cells that did not infiltrate or injure the grafts up to 28 days after adoptive transfer. Administration of porcine interferon-gamma for up to 28 days sustained the expression of graft vascular cell adhesion molecule-1 and major histocompatibility complex antigens, but did not initiate recruitment of human leukocytes. In contrast, treatment with human tumor necrosis factor for 7 days induced the de novo expression of porcine E-selectin by graft endothelial cells and elicited human T cell infiltration and human peripheral blood mononuclear cell-dependent vascular injury.The human peripheral blood mononuclear cell-severe combined immunodeficiency/beige mouse model identifies a significant difference between human T cell allogeneic and xenogeneic responses in vivo. Xenografts with quiescent endothelium are not infiltrated or injured by T cells under the same conditions in which allografts are rejected. Activation of pig coronary artery endothelial cells by human tumor necrosis factor, but not porcine interferon-gamma, elicits cellular xenoresponses.
科研通智能强力驱动
Strongly Powered by AbleSci AI