介电谱
聚苯胺
生物传感器
循环伏安法
微分脉冲伏安法
材料科学
寡核苷酸
纳米复合材料
DNA
电化学
检出限
纳米技术
化学
电极
色谱法
聚合物
生物化学
复合材料
物理化学
聚合
作者
Razieh Salimian,Saeed Shahrokhian,Saba Panahi
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2019-03-21
卷期号:5 (5): 2587-2594
被引量:27
标识
DOI:10.1021/acsbiomaterials.8b01520
摘要
Herein, we present a novel, simple, and ultrasensitive electrochemical DNA (E-DNA) sensor based on hollow carbon spheres (HCS) decorated with polyaniline (PANI). A thiolated 21-mer oligonucleotide, characteristic of HBV DNA, is immobilized via electrodeposited gold nanoparticles on HCS-PANI. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) are used to characterize the electrochemical properties of the prepared nanocomposite. Scanning electron microscopy is employed to investigate the morphological texture of the fabricated modifier. An enhanced intrinsic signal of PANI is probed to evaluate the biosensing ability of the prepared modifier. The proposed biosensor allows for the detection of the target sequences of HBV DNA at a concentration as low as 10 fM (i.e., 109 DNA copies/mL). In addition, this biosensor demonstrated good capability to differentiate between the perfectly matched target oligonucleotide and three nucleotide-mismatched oligonucleotides. Furthermore, the HCS/PANI-based E-DNA sensor indicates highly sensitive detection of HBV DNA in real samples.
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