DNA Isolation by Lysozyme and Proteinase K

AbstractThe isolation of DNA using lysozyme and Proteinase K is suitable for extraction of DNA from microorganisms particularly Gram-positive bacteria. The application of enzymes like lysozyme and Proteinase K at suitable temperatures, duration, and pH helps in the degradation of hard and rigid peptidoglycan cell walls of these microorganisms. Therefore, it is the most efficient and quick method for the isolation of DNA from microorganisms without using harsh biochemicals. However, further purification of DNA can be required by other methods for utilizing it further for molecular studies. This method is widely used in microbiology research, production of recombinant DNAs, biotechnology-based industries, and medical diagnosis where the presence of microbial DNA confirms the pathogenicity of a particular disease.KeywordsDNAMicroorganismsGram-positive bacteriaProteinase KLysozyme