Anti‐hyperglycemia effect of Lactobacillus reuteri GMNL‐89 throught modulating of PPARs and Energy Metabolism Related Genes expression in Insulin‐Resistant Rats

The present study was designed to understand the effect of oral administration Lactobacillus reuteri GMNL-89 (Lr 89) on hyperglycemia and related gene expression by using STZ and high fat diet to induce SD rat with insulin resistance. 4 groups of 40 Sprague-Dawley male rats were fed for 8 weeks with (1) control group (Control ; C), (2) insulin resistance rats given ddH2O group (Insulin Resistance; IR),(3) insulin resistance rats given 1.6x108 CFU/ml of Lr 89 group (IR+1.6x108 CFU Lr 89; 1X), and (4)insulin resistance rats given 8x108 CFU/ml of Lr 89 group (IR+8x108 CFU Lr 89; 5X). Insuline resistance was induced by chronic low does STZ injection and a high fat diet to mimic insulin resistance. Oral glucose tolerance test (OGTT), fasting blood sugar (FBS), and fasting insulin were tested, and the live tissues were collected to analyze the gene expression of PPARs, SREPB-1,ChREBP, FAS, and Cyp7a1 by real-time PCR. The level of OGTT, FBS, fasting insulin was significantly lower in 1x, 5x groups than the IR group (P<0.05). The results of real-time PCR showed that the expressions of PPARα, PPARγ, and Cyp7a1 in supplemental 1x and 5x groups were higher than the IR group (P<0.05). In contrast, the level of ChREBP and FAS was significantly lower in 1X and 5X groups than the IR group (P<0.05). The antihyperglycemic property of Lactobacillus reuteri GMNL-89 perhaps increase PPARs, SREPB-1, ChREBP, FAS, and CYP7A1gene expression. Therefore, oral Lr 89 can be considered as an alternative medicine to treat hyperglycemia in insulin resistance rat.