硫代葡萄糖苷
代谢组
类黄酮
转录组
硒酸盐
萝卜硫苷
食品科学
硒
化学
生物
生物化学
抗氧化剂
芸苔属
植物
基因
代谢物
基因表达
有机化学
作者
Shen Rao,Yuanyuan Gou,Yu Tian,Xin Cong,Jia-Ying Gui,Zhenzhou Zhu,Weiwei Zhang,Yongling Liao,Jiabao Ye,Shuiyuan Cheng,Feng Xu
标识
DOI:10.1016/j.foodres.2021.110463
摘要
Broccoli is a nutritious vegetable popular all over the world. This study investigated the effects of different concentrations of selenate (0, 0.1, 0.2, 0.4, 0.8, and 1.6 mmol/L) on the selenium (Se), glucosinolate, and flavonoid contents of broccoli florets. Results showed that the total Se, selenomethionine, and methyl selenocysteine contents increased following selenate dosage. Interestingly, selenate treatment of 0.4 mmol/L decreased the flavonoid but increased the glucosinolate content. Metabolome analysis revealed changes in the individual contents of glucosinolates and flavonoids. Conjoint analysis of transcriptome and metabolome showed that the glucosinolate and flavonoid compounds were potentially regulated by two sulfate transporter genes (Sultr3;1 and Sultr4;2) and several cytochrome P450 genes (e.g., CYP71B21, CYP72C1, and CYP81F1). These new findings indicated that Se treatment may influence glucosinolate and flavonoid accumulation by regulating the expression of these genes. The results of this study provide some novel insights into the effects of Se on glucosinolates and flavonoids in broccoli florets and deepen our understanding of the regulatory network between some specific genes and these compounds.
科研通智能强力驱动
Strongly Powered by AbleSci AI