竞争性内源性RNA
癌症研究
肝细胞癌
免疫印迹
反义RNA
分子生物学
细胞生长
细胞培养
生物
核糖核酸
小RNA
长非编码RNA
基因
生物化学
遗传学
作者
Jing-Hua Kuai,Zheng Li,Xin Yi,Zengli Liu,Bo Qiu,Zhihua Lu,Yuanhui Jiang
标识
DOI:10.1016/j.dld.2021.02.012
摘要
Increasing studies have shown a vital fact that long non-coding RNAs (lncRNAs) play a considerable regulatory role in hepatocellular carcinoma (HCC) progression. However, whether ST8 alpha-N-acetyl-neuraminide alpha-2, 8-sialyltransferase 6 antisense RNA 1 (ST8SIA6-AS1) affects the development of HCC is unclear.The target genes in HCC cell lines were quantified via utilzing quantitative real-time polymerase chain reaction (RT-qPCR) analysis and western blot. Effects of ST8SIA6-AS1 on proliferative, apoptosis and migratory ability of HCC cells were proved by a series of function experiments. The cellular distribution of ST8SIA6-AS1 was examined through fluorescent in situ hybridization (FISH) assay and subcellular fractionation experiments. RNA pulldown assay was implemented to explore the target of ST8SIA6-AS1. RNA Binding Protein Immunoprecipitation (RIP) and luciferase reporter assays were performed to identify the specific relationships between miR-338-3p and ST8SIA6-AS1/ non-POU domain containing octamer binding (NONO).The expression of ST8SIA6-AS1 was apparently elevated in HCC cell. Silenced ST8SIA6-AS1 reduced proliferative, migratory and invasive ability of HCC cells. Moreover, ST8SIA6-AS1 targeted miR-338-3p to modulate the expression of NONO in HCC cells.ST8SIA6-AS1 enhances the progression of HCC via miR-338-3p/NONO axis in vitro.
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