光度计
光密度
校准
细胞计数
显微镜
材料科学
电阻器
光学
生物系统
光电子学
化学
数学
生物
细胞
物理
工程类
电气工程
统计
电压
生物化学
细胞周期
作者
Piyachat Wungmool,Nattha Rangsi,Tongchai Hormwantha,Malee Sutthiopad,Chaiya Luengviriya
出处
期刊:Journal of physics
[IOP Publishing]
日期:2019-08-01
卷期号:1298 (1): 012005-012005
被引量:2
标识
DOI:10.1088/1742-6596/1298/1/012005
摘要
Abstract Microalgae are currently interesting in industry because it can generate many useful products, such as supplementary food, cosmetic and biomass energy. During the cultivation, the growth of microalgae is traditionally followed by cell counting with an optical microscope and a calculation of the cell density. To obtain a precise value of the cell density, the measurement should be performed by ones with good laboratory skills and it takes a long time. We develop a photometer to determine the cell density of microalgae. The photometer consists of a light dependent resistor as the sensor, a light emitting diode as the light source, and a microcontroller which controls all components as well as the cell density calculation using a calibration equation. To test the performance of our device, we cultivate marine microalgae Dunaliella Salina with different aerations and measure the cell density during 18-day cultivation period by using the photometer and the cell counting with an optical microscope. The results show that the cell density values obtained from both measurement methods increase with day of cultivation in a similar manner. Thus, the photometer is a good alternative device to follow the growth of microalgae. Furthermore, it is relatively inexpensive, quick and easy for the operator to use.
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