生物传感器
噬菌体
检出限
材料科学
金黄色葡萄球菌
戊二醛
连接器
线性范围
细菌
纳米技术
色谱法
化学
生物
大肠杆菌
生物化学
操作系统
基因
遗传学
计算机科学
作者
Neha Bhardwaj,Sanjeev K. Bhardwaj,Jyotsana Mehta,Ki‐Hyun Kim,Akash Deep
标识
DOI:10.1021/acsami.7b07818
摘要
To produce a sensitive and specific biosensor for Staphylococcus aureus, bacteriophages have been interfaced with a water-dispersible and environmentally stable metal–organic framework (MOF), NH2-MIL-53(Fe). The conjugation of the MOF with bacteriophages has been achieved through the use of glutaraldehyde as cross-linker. Highly sensitive detection of S. aureus in both synthetic and real samples was realized by the proposed MOF–bacteriophage biosensor based on the photoluminescence quenching phenomena: limit of detection (31 CFU/mL) and range of detection (40 to 4 × 108 CFU/mL). This is the first report exploiting the use of an MOF–bacteriophage complex for the biosensing of S. aureus. The results of our study highlight that the proposed biosensor is more sensitive than most of the previous methods while exhibiting some advanced features like specificity, regenerability, extended range of linear detection, and stability for long-term storage (even at room temperature).
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