血凝素(流感)
荧光
化学
检出限
肉眼
病毒
甲型流感病毒
禽流感病毒
病毒学
H5N1亚型流感病毒
生物
色谱法
量子力学
物理
作者
Xiaoling Pan,Pai Liu,Xinghui Wu,Yahui Zhang,Zhengxu Cai,Jianbing Shi,Junge Zhi,Zi Li,Dayang Wang,Bin Tong,Yuping Dong
标识
DOI:10.1016/j.snb.2021.130392
摘要
Four hexaphenyl-butadiene pyridine salt derivatives (HPB-Xs) with aggregation-induced emission (AIE) characteristics were designed and synthesized. After comprehensive comparison of the four compounds, HPB-I was selected for protein detection. The good-linear response of HPB-I to hemagglutinin 5 protein (H5) on surface of influenza virus were realized with the characteristics of real-time, high-selective, and sensitive in a wide range of H5 concentration. The limit of detection (LOD) was as low as 179.5 ng/mL (3.04 nM) in throat swaps. HPB-I also achieved colorimetric detection for H5 by naked eyes. The simulation calculations based on molecular docking revealed that HPB-I could well combine with the top-cavity of H5 and achieved single-molecule-limited “turn-on” fluorescence signal. This study is a significant step in the development of a low-cost influenza test at the point of pursuing a new strategy for the molecular design of antiviral drugs or virus staining and tracing.
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