过氧化氢
生物传感器
电化学
检出限
材料科学
Nafion公司
传统PCI
心肌梗塞
生物医学工程
色谱法
纳米技术
电极
化学
医学
内科学
有机化学
物理化学
作者
Wei Xu,Marwan Sakran,Jianwen Fei,Xiaoyun Li,Chenyuan Weng,Wei Yang,Guomin Zhu,Wanying Zhu,Xuemin Zhou
出处
期刊:ACS Biomaterials Science & Engineering
[American Chemical Society]
日期:2021-05-04
卷期号:7 (6): 2767-2773
被引量:22
标识
DOI:10.1021/acsbiomaterials.1c00242
摘要
Hydrogen peroxide (H2O2) has been reported to mediate a variety of physiological and pathological processes in living systems. In this work, a biosensor for determination of H2O2 was prepared by using an HRP/Ti3C2/Nafion film-modified glassy carbon electrode (GCE). Ti3C2 nanosheets with remarkable conductivity and high specific surface area were chosen as carriers for HRP. Moreover, this biosensor modified with HRP has a specific catalytic effect on H2O2. The difference in peak current could reflect the quantitative change of H2O2. The linear range of the biosensor is 5–8000 μM, and the detection limit is 1 μM (S/N = 3). This biosensor was used to detect H2O2 in clinical serum samples of normal controls and patients with acute myocardial infarction (AMI) before and after percutaneous coronary intervention (PCI). The results showed that the difference between normal controls and patients is significant (P < 0.05), as well as the difference for patients before and after PCI (P < 0.01), but no significant difference existed between postoperative patients and normal controls. This biosensor has the advantages of simple preparation, high sensitivity, and quick detection, showing potential application in clinical diagnosis.
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