整合素
粘附
配体(生物化学)
细胞粘附
纳米棒
纳米尺度
荧光各向异性
材料科学
生物物理学
各向异性
纳米技术
巨噬细胞极化
化学
细胞生物学
巨噬细胞
受体
生物
体外
生物化学
光学
物理
复合材料
膜
作者
Heemin Kang,Siu Hong Dexter Wong,Qi Pan,Gang Li,Liming Bian
出处
期刊:Nano Letters
[American Chemical Society]
日期:2019-02-11
卷期号:19 (3): 1963-1975
被引量:46
标识
DOI:10.1021/acs.nanolett.8b05150
摘要
Material implants trigger host reactions generated by cells, such as macrophages, which display dynamic adhesion and polarization including M1 inflammatory state and M2 anti-inflammatory state. Creating materials that enable diverse nanoscale display of integrin-binding groups, such as RGD ligand, can unravel nanoscale recruitment and ligation of integrin, which modulate cellular adhesion and activation. Here, we synthesized gold nanorods (GNRs) with various nanoscale anisotropies (i.e., aspect ratios, ARs), but in similar surface areas, and controlled their substrate conjugation to display an anisotropic ligand nanogeometry without modulating ligand density. Using nanoscale immunolabeling, we demonstrated that highly anisotropic ligand-coated GNRs ("AR4" and "AR7") facilitated the recruitment of integrin β1 on macrophages to their nanoscale surfaces. Consequently, highly anisotropic GNRs (e.g., "AR4" and "AR7") elevated the adhesion and M2 state of macrophages, with the inhibition of their M1 state in the culture and mice, entailing rho-associated protein kinase. This nanoscale anisotropic nanogeometry provides a novel and critical parameter to be considered in the generation of biomaterials to potentially modulate host reactions to the implants for immunomodulatory tissue regeneration.
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