The development and properties of nanoemulsions stabilized with glycated soybean protein for carrying β-carotene

Plant protein nanoemulsion delivery system has high biocompatibility, but its instability in special environment needs to be solved urgently. In this study, glycated soybean protein isolate (GSPI) was used to the construction of nanoemulsions for carrying β-carotene. The interface properties of GSPI, the stability and digestive properties of nanoemulsions stabilized by GSPI were investigated. The results showed that the diffusion rate of GSPI at oil-water interface decreased, while the permeation and rearrangement rates increased. Moreover, the interfacial film formed by GSPI had higher viscoelasticity. The average particle size of GSPI nanoemulsions was less than 500 nm, and the retention rate of β-carotene was more than 80% during the storage of 28 days. Meanwhile, the bioaccessibility of β-carotene in GSPI nanoemulsions increased to above 70% after in vitro digestion. It is concluded that glycated soybean protein nanoemulsions have good storage stability and can be used to encapsulate and deliver of β-carotene.