Process development for purification of phospholipids by alumina column chromatography

A process for simultaneous purification of phosphatidyl choline(PC) and phosphatidyl ethanolamine(PE) by alumina column chromatography was developed,and the optimization of operating parameters was made.Better operating parameters for loading,eluting and regenerating were as follows: for loading,pure methanol as mobile phase,flow rate of 1.0-2.5 BV/h and sample loading concentration of 100 g/L;for eluting,chloroform-methanol as an eluent in which chloroform volume fraction was 80%;for regenerating,washing with methanol for 5 bed volumes.The feasibility of the process was proved with continuous loading experiments.In the process,PC and PE with purities over 95% and 80% respectively could be obtained.The process features large loading quantity,high yield and purity of PC and PE.