Assessment of whitening efficacy of cosmetical materials in B16 melanoma cells

Objective To assess the whitening efficacy of cosmetical materials in B16 melanoma cells.Methods B16 melanoma cells were cultured in vitro,and the inhibition of cosmetical materials on the melanogenesis was measured with L-Dopa dying and mean optical density method.Results Melanin could be dyed by L-Dopa specifically in B16 melanoma cells,and the inhibition of Kojic acid with concentration of 25μg/ml on the melanogenesis was(48.44±3.44)%,and the RSD within and between the plate were 7.09% and 7.22%,respectively.Kojic acid,Resveratrol and Camellia sinensis extract could inhibit the melanogenesis effectively,while White mulberry root-bark extract didn't have any inhibition effect on the melanogenesis.Conclusions The content of melanin in B16 melanoma cells was measured with L-Dopa dying and mean optical density method,which is proven to be higher sensitivity and lower error,and could be used for the assessment of cosmetical materials on the whitening efficacy successfully.