环己酰亚胺
伤口愈合
三氯乙酸
生物化学
蛋白质生物合成
赤霉素
生长素
赤霉素
过氧化物酶
亮氨酸
生物
化学
氨基酸
酶
植物
发芽
基因
免疫学
作者
Rolf Borchert,James D. McChesney,David M. Watson
摘要
Several aspects of wound healing in tuber tissue of potato (Solanum tuberosum var. Kennebec), known to require protein synthesis, are inhibited by 2,4-dichlorobenzyltributylphosphonium chloride (Phosphon D). Cell division was completely blocked by 60 mum Phosphon and markedly reduced by concentrations as low as 3 mum. When applied at the time of wounding, 0.25mm Phosphon completely prevented the wound-induced respiratory increase. Application at 15 hours after wounding arrested respiration at the rate present at that time. The same concentrations of Phosphon inhibited auxin-induced cell expansion of the tissue, protein synthesis as measured by the incorporation of leucine-(14)C into the trichloroacetic acid-insoluble fraction of tissue disks, and the appearance of wound-induced peroxidase isozymes. None of these inhibitory effects of Phosphon could be prevented or reversed by the application of gibberellic acid. All wound-induced processes inhibited by Phosphon are also inhibited by cycloheximide. It is suggested that inhibitory effects of Phosphon on wound healing in potato and on other developmental processes in excised plant tissues which cannot be reversed by gibberellin are due to interference with protein synthesis.
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