化学
多酚
核磁共振波谱
分子间力
动态光散射
分子动力学
蛋白质-蛋白质相互作用
三元络合物
生物物理学
生物化学
计算化学
分子
立体化学
纳米技术
有机化学
酶
抗氧化剂
材料科学
纳米颗粒
生物
作者
Benoit Faurie,Érick J. Dufourc,Michel S. Laguerre,Isabelle Pianet
标识
DOI:10.1021/acs.analchem.6b03911
摘要
Gaining insight into intermolecular interactions between multiple species is possible at an atomic level by looking at different parameters using different NMR techniques. In the specific case of the astringency sensation, in which at least three molecular species are involved, different NMR techniques combined with dynamic light scattering and molecular modeling contribute to decipher the role of each component in the interaction mode and to assess the thermodynamic parameters governing this complex interaction. The binding process between a saliva peptide, a polyphenol, and polysaccharides was monitored by following 1H chemical shift variations, changes in NMR peak areas, and size of the formed complex. These NMR experiments deliver a complete picture of the association pathway, assessed by dynamic light scattering and molecular dynamics simulations: all of the data collected converge toward a comprehensive mode of interaction in which sugars indirectly play a role in astringency by sequestering part of the polyphenols, reducing their effective concentration to bind saliva proteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI