人血清白蛋白
化学
傅里叶变换红外光谱
范德瓦尔斯力
氢键
荧光
圆二色性
纳米颗粒
蛋白质二级结构
分析化学(期刊)
生物物理学
结晶学
分子
纳米技术
色谱法
材料科学
有机化学
生物化学
化学工程
生物
物理
量子力学
工程类
出处
期刊:Luminescence
[Wiley]
日期:2020-07-01
卷期号:35 (8): 1269-1276
摘要
Abstract Techniques such as Fourier transform infrared (FTIR), ultraviolet–visible (UV–vis) spectra, fluorescence, circular dichroism (CD) and spectroscopy were applied to elucidate the formation, structure and physicochemical properties of levobupivacaine–gold nanoparticle (LGN) binding to human serum albumin (HSA). Thermodynamic parameters (Δ G = −2.58 × 10 4 J·mol −1 , Δ S = −7.80 J·mol −1 ·K −1 , and Δ S = −2.82 × 10 4 J·mol −1 at 305 K) suggested one weak binding site on HSA, which was governed by van der Waals forces as well as hydrogen bonds. Moreover, the outcomes of UV–vis, CD, FTIR, synchronous and three‐dimensional fluorescence suggested that the microenvironment of HSA had been changed with addition of LGN. Based on the results of fluorescence resonance energy transfer, a distance of 2.8 nm between the LGN and HSA was observed. This approach has potential value for illustrating the pharmacodynamics of LGN when in combination with transmembrane transport, biomolecular function effect, and other experiments.
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