The air parameters of fine manipulation room were assayed.The results were as follows: The new wind quantity of air conditioner room was not adequate and its CO 2 content was also higher than natural fine manipulation room; the distribution of the air parameters such as temperature, relative humidity, airflow speed and CO 2 content was uneven; the type of delivering wind affected the airflow form. It was put forward that the airflow factors of fine manipulation room such as new wind quantity, airflow speed and the airflow form greatly affected environment of fine manipulation.