脂质代谢
蛋白质组学
串联质量标签
脑脊液
等压标记
载脂蛋白A1
载脂蛋白E
生物
结核性脑膜炎
结核分枝杆菌
定量蛋白质组学
肺结核
新陈代谢
折叠变化
免疫学
基因本体论
载脂蛋白B
医学
基因表达
生物化学
基因
病理
胆固醇
疾病
神经科学
作者
Jun Mu,Yongtao Yang,Jin Chen,Ke Cheng,Qi Li,Yongdong Wei,Dan Zhu,Wei Shao,Peng Zheng,Peng Xie
标识
DOI:10.1016/j.bbrc.2015.08.036
摘要
Tuberculous meningitis (TBM) remains to be one of the most deadly infectious diseases. The pathogen interacts with the host immune system, the process of which is largely unknown. Various cellular processes of Mycobacterium tuberculosis (MTB) centers around lipid metabolism. To determine the lipid metabolism related proteins, a quantitative proteomic study was performed here to identify differential proteins in the cerebrospinal fluid (CSF) obtained from TBM patients (n = 12) and healthy controls (n = 12). CSF samples were desalted, concentrated, labelled with isobaric tags for relative and absolute quantitation (iTRAQ™), and analyzed by multi-dimensional liquid chromatography-tandem mass spectrometry (LC-MS/MS). Gene ontology and proteomic phenotyping analysis of the differential proteins were conducted using Database for Annotation, Visualization, and Integrated Discovery (DAVID) Bioinformatics Resources. ApoE and ApoB were selected for validation by ELISA. Proteomic phenotyping of the 4 differential proteins was invloved in the lipid metabolism. ELISA showed significantly increased ApoB levels in TBM subjects compared to healthy controls. Area under the receiver operating characteristic curve analysis demonstrated ApoB levels could distinguish TBM subjects from healthy controls and viral meningitis subjects with 89.3% sensitivity and 92% specificity. CSF lipid metabolism disregulation, especially elevated expression of ApoB, gives insights into the pathogenesis of TBM. Further evaluation of these findings in larger studies including anti-tuberculosis medicated and unmedicated patient cohorts with other center nervous system infectious diseases is required for successful clinical translation.
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