拟南芥
转基因作物
转基因
耐旱性
非生物胁迫
生物
调节器
拟南芥
非生物成分
脱落酸
蔗糖
细胞生物学
基因
植物
生物化学
突变体
古生物学
作者
Yongheng Zhang,Siqin Wan,Xianghong Liu,Jingyuan He,Long Cheng,Minghui Duan,Huan Liu,Weidong Wang,Youben Yu
标识
DOI:10.1016/j.plaphy.2020.02.035
摘要
Drought is a major factor limiting crop productivity and quality. Sucrose non-fermenting-1 (SNF1)-related protein kinase 2s (SnRK2s) play critical roles in plant abiotic stress responses, especially in drought stress. However, knowledge regarding the functional roles of SnRK2s in drought stress and their underlying mechanisms is relatively limited in tea plant. In this study, CsSnRK2.5, a PEG 6000- and ABA-induced SnRK2 gene from tea plant, was overexpressed in Arabidopsis to investigate its potential function in drought stress response. The results showed that overexpression of CsSnRK2.5 resulted in enhanced drought tolerance, as indicated by an amelioration of the changes in various physiological indexes, including a decreased rate of water loss and decreased accumulation of ROS and MDA. In addition, CsSnRK2.5 overexpression conferred hypersensitivity to exogenous ABA, and transgenic plants exhibited improved ABA-mediated stomatal closure compared to WT plants. Moreover, the expression of some stress response genes, including AtRAB18 and AtRD29b, was more strongly induced in transgenic plants than in the WT when subjected to ABA and drought treatments. Taken together, our results indicate that CsSnRK2.5 is a positive regulator of ABA-regulated drought stress responses.
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