素数(序理论)
基因组编辑
计算机科学
清脆的
计算生物学
基因
生物
遗传学
数学
组合数学
作者
Yuanyuan Jiang,Yiping Chai,Minhui Lu,Xiuli Han,Yu Zhang,Qiang Zhang,Yun Zhou,Xuechen Wang,Qijun Chen
标识
DOI:10.1101/2020.07.06.188896
摘要
Abstract A novel and universal CRISPR/Cas-derived precision genome-editing technology named prime editing was developed. However, low efficiency of prime editing was shown in transgenic rice lines. We reasoned that enhancing pegRNA expression would improve prime-editing efficiency. In this report, we describe two strategies for enhancing pegRNA expression. We constructed a prime-editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2 . Compared with previous reports in rice, we achieved much higher prime-editing efficiency in maize. Our results are inspiring and provide a direction for the optimization of plant prime editors.
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