坏死性下垂
裂谷1
程序性细胞死亡
炎症
过渡(遗传学)
肿瘤坏死因子α
调解人
细胞凋亡
胞浆
交通2
细胞生物学
信号转导
生物
化学
癌症研究
免疫学
肿瘤坏死因子受体
生物化学
酶
作者
Swati Choksi,Gourav Choudhary,Zheng-gang Liu
出处
期刊:Methods in molecular biology
日期:2020-11-14
卷期号:: 73-80
被引量:2
标识
DOI:10.1007/978-1-0716-1130-2_5
摘要
Tumor necrosis factor (TNF) plays a key role in inflammatory responses and in various cellular events such as apoptosis and necroptosis. The interaction of TNF with its receptor, TNFR1, drives the initiation of complex molecular pathways leading to inflammation and cell death. RARγ is released from the nucleus to orchestrate the formation of the cytosolic death complexes, and it is cytosolic RARγ that plays a pivotal role in switching TNF-induced inflammatory responses to RIPK1-initiated cell death. Thus, RARγ provides a checkpoint for the transition from inflammatory signaling to death machinery of RIPK1-initiated cell death in response to TNF. Here, we use techniques to identify RARγ as a downstream mediator of TNFR1 signaling complex. We use confocal imaging to show the localization of RARγ upon activation of cell death. Immunoprecipitation of RARγ identified the interacting proteins.
科研通智能强力驱动
Strongly Powered by AbleSci AI