蛋白质生物合成
翻译(生物学)
信使核糖核酸
抄写(语言学)
核糖核酸
生物化学
生物
计算生物学
细胞生物学
基因
语言学
哲学
作者
Joseph Yang,Yong Han,Jongwon Im,Sang Woo Seo
标识
DOI:10.1038/s41589-021-00736-3
摘要
Coupled transcription and translation processes in bacteria cause indiscriminate translation of intact and truncated messenger RNAs, inevitably generating nonfunctional polypeptides. Here, we devised a synthetic protein quality control (ProQC) system that enables translation only when both ends of mRNAs are present and followed by circularization based on sequence-specific RNA-RNA hybridization. We demonstrate that the ProQC system dramatically improved the fraction of full-length proteins among all synthesized polypeptides by selectively translating intact mRNA and reducing abortive translation. As a result, full-length protein synthesis increased up to 2.5-fold without changing the transcription or translation efficiency. Furthermore, we applied the ProQC system for 3-hydroxypropionic acid, violacein and lycopene production by ensuring full-length expression of enzymes in biosynthetic pathways, resulting in 1.6- to 2.3-fold greater biochemical production. We believe that our ProQC system can be universally applied to improve not only the quality of recombinant protein production but also efficiencies of metabolic pathways.
科研通智能强力驱动
Strongly Powered by AbleSci AI