Observation of autophagy during scrap Leather–Induced neuroinflammation via lysosome-targeting polarity-specific two-photon probe

Autophagy is a protective mechanism that exists in cells and plays an important role in maintaining the normal physiological morphology of cells and in the disease regulation of living organisms. Recent studies have shown that changes in lysosomal polarity can be an important indicator for assessing cellular autophagy. Thus, we developed a probe (Lyso-Pola) that is sensitive to lysosomal polarity to monitor these changes in order to assess the extent of cellular autophagy during scrap leather–induced neuroinflammation. The relationship between cellular autophagy, neuroinflammation, and scrap leather residues was also explored. The experimental results showed that the fluorescence signal of Lyso-Pola was significantly lower during cellular autophagy in two-photon confocal imaging. In addition, models of scrap leather–induced neuroinflammation in BV2 cells and mice showed that neuroinflammation can lead to the release of reactive oxygen species, which then induces cellular autophagy.