生物
霍乱毒素
生物化学
内吞作用
内体
效应器
细胞内
作者
Aftab Nadeem,Athar Alam,Eric Toh,Si Lhyam Myint,Zia Ur Rehman,Tao Liu,Marta Bally,Anna Arnqvist,Hui Wang,Jun Zhu,Karina Persson,Bernt Eric Uhlin,Sun Nyunt Wai
出处
期刊:PLOS Pathogens
[Public Library of Science]
日期:2021-03-18
卷期号:17 (3)
被引量:7
标识
DOI:10.1371/journal.ppat.1009414
摘要
Vibrio cholerae is a noninvasive intestinal pathogen extensively studied as the causative agent of the human disease cholera. Our recent work identified MakA as a potent virulence factor of V. cholerae in both Caenorhabditis elegans and zebrafish, prompting us to investigate the potential contribution of MakA to pathogenesis also in mammalian hosts. In this study, we demonstrate that the MakA protein could induce autophagy and cytotoxicity of target cells. In addition, we observed that phosphatidic acid (PA)-mediated MakA-binding to the host cell plasma membranes promoted macropinocytosis resulting in the formation of an endomembrane-rich aggregate and vacuolation in intoxicated cells that lead to induction of autophagy and dysfunction of intracellular organelles. Moreover, we functionally characterized the molecular basis of the MakA interaction with PA and identified that the N-terminal domain of MakA is required for its binding to PA and thereby for cell toxicity. Furthermore, we observed that the ΔmakA mutant outcompeted the wild-type V. cholerae strain A1552 in the adult mouse infection model. Based on the findings revealing mechanistic insights into the dynamic process of MakA-induced autophagy and cytotoxicity we discuss the potential role played by the MakA protein during late stages of cholera infection as an anti-colonization factor.
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