材料科学
夹层盘
心肌细胞
污渍
细胞生物学
整合素
碳纳米管
心肌细胞
细胞内
缝隙连接
生物物理学
下调和上调
纳米技术
化学
生物
细胞
生物化学
基因
作者
Haiyan Sun,Shuaiyao Lu,Xiaoxia Jiang,Xia Li,Hong Li,Qiuxia Lin,Yongchao Mou,Yuwei Zhao,Yifeng Han,Jin Zhou,Changyong Wang
出处
期刊:Biomaterials
[Elsevier]
日期:2015-07-01
卷期号:55: 84-95
被引量:67
标识
DOI:10.1016/j.biomaterials.2015.03.030
摘要
Carbon nanotubes (CNTs) offer a new paradigm for constructing functional cardiac patches and repairing myocardial infarction (MI). However, little is known about how CNTs enhance the mechanical integrity and electrophysiological function of cardiac myocytes. To address this issue, we investigated the regularity and precise mechanism of the influence of CNTs on the assembly of intercalated disc (IDs). Here, single walled CNTs incorporated into collagen substrates were utilized as growth supports for neonatal cardiomyocytes, which enhanced cardiomyocyte adhesion and maturation. Furthermore, through the use of immunohistochemical staining, western blotting, transmission electron microscopy, and intracellular calcium transient measurement, we discovered that the addition of CNTs remarkably increased ID-related protein expression and enhanced ID assembly and functionality. On that basis, we further explored the underlying mechanism for how CNTs enhanced ID assembly through the use of immunohistochemical staining and western blotting. We found that the β1-integrin-mediated signaling pathway mediated CNT-induced upregulation of electrical and mechanical junction proteins. Notably, CNTs remarkably accelerated gap junction formation via activation of the β1-integrin-mediated FAK/ERK/GATA4 pathway. These findings provide valuable insight into the mechanistic effects that CNTs have on neonatal cardiomyocyte performance and will have a significant impact on the future of nanomedical research.
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