交易激励
转录因子
Ccaat增强子结合蛋白
生物
脂肪生成
P300-CBP转录因子
分子生物学
激活转录因子
乙酰化
抄写(语言学)
细胞生物学
DNA结合蛋白
基因
生物化学
哲学
间充质干细胞
组蛋白乙酰转移酶
语言学
作者
Yue Zhao,Ya Dong Zhang,You You Zhang,Shu Wen Qian,Zhi Chun Zhang,Shu Fen Li,Liang Guo,Yuan Liu,Bo Wen,Qun-Ying Lei,Qi Tang,Xi Li
摘要
Adipogenesis is a multistep process by which 3T3-L1 preadipocytes differentiate into mature adipocytes through mitotic clonal expansion (MCE) and terminal differentiation.The CCAAT/enhancer-binding protein  (C/EBP) is an important transcription factor that takes part in both of these processes.C/EBP not only transactivates C/EBP␣ and the peroxisome proliferatoractivated receptor ␥ (PPAR␥), which cause 3T3-L1 preadipocytes to enter terminal adipocyte differentiation, but also is required to activate cell cycle genes necessary for MCE.The identification of potential cofactors of C/EBP will help to explain how C/EBP undertakes these specialized roles during the different stages of adipogenesis.In this study, we found that activating transcription factor 5 (ATF5) can bind to the promoter of C/EBP␣ via its direct interaction with C/EBP (which is mediated via the p300-dependent acetylation of ATF5), leading to enhanced C/EBP transactivation of C/EBP␣.We also show that p300 is important for the interaction of ATF5 with C/EBP as well as for the binding activity of this complex on the C/EBP␣ promoter.Consistent with these findings, overexpression of ATF5 and an acetylated ATF5 mimic both promoted 3T3-L1 adipocyte differentiation, whereas short interfering RNA-mediated ATF5 downregulation inhibited this process.Furthermore, we show that the elevated expression of ATF5 is correlated with an obese phenotype in both mice and humans.In summary, we have identified ATF5 as a new cofactor of C/EBP and examined how C/EBP and ATF5 (acetylated by a p300-dependent mechanism) regulate the transcription of C/EBP␣.
科研通智能强力驱动
Strongly Powered by AbleSci AI