生物
泛素连接酶
核糖核酸
泛素
细胞生物学
绑定域
非编码RNA
RNA结合蛋白
结合位点
生物化学
基因
作者
Nila Roy Choudhury,Gregory Heikel,Maryia Trubitsyna,Peter Kubík,Jakub Stanislaw Nowak,Shaun Webb,Sander Granneman,Christos Spanos,Juri Rappsilber,Alfredo Castelló,Gracjan Michlewski
出处
期刊:BMC Biology
[Springer Nature]
日期:2017-11-08
卷期号:15 (1)
被引量:122
标识
DOI:10.1186/s12915-017-0444-9
摘要
TRIM25 is a novel RNA-binding protein and a member of the Tripartite Motif (TRIM) family of E3 ubiquitin ligases, which plays a pivotal role in the innate immune response. However, there is scarce knowledge about its RNA-related roles in cell biology. Furthermore, its RNA-binding domain has not been characterized.Here, we reveal that the RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain, which we postulate to be a novel RNA-binding domain. Using CLIP-seq and SILAC-based co-immunoprecipitation assays, we uncover TRIM25's endogenous RNA targets and protein binding partners. We demonstrate that TRIM25 controls the levels of Zinc Finger Antiviral Protein (ZAP). Finally, we show that the RNA-binding activity of TRIM25 is important for its ubiquitin ligase activity towards itself (autoubiquitination) and its physiologically relevant target ZAP.Our results suggest that many other proteins with the PRY/SPRY domain could have yet uncharacterized RNA-binding potential. Together, our data reveal new insights into the molecular roles and characteristics of RNA-binding E3 ubiquitin ligases and demonstrate that RNA could be an essential factor in their enzymatic activity.
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