纳米地形
细胞生物学
间充质干细胞
焦点粘着
再生(生物学)
丝状体
细胞外基质
材料科学
脚手架
骨愈合
生物医学工程
化学
信号转导
解剖
生物
肌动蛋白
医学
作者
Liang Ma,Wencan Ke,Zhiwei Liao,Xiaobo Feng,Jie Lei,Kun Wang,Bingjin Wang,Gaocai Li,Rongjin Luo,Shi Yunsong,Weifeng Zhang,Yu Song,Weibin Sheng,Cao Yang
标识
DOI:10.1016/j.bioactmat.2022.01.008
摘要
Nanotopographical cues endow biomaterials the ability to guide cell adhesion, proliferation, and differentiation. Cellular mechanical memory can maintain the cell status by retaining cellular information obtained from past mechanical microenvironments. Here, we propose a new concept "morphology memory of small extracellular vesicles (sEV)" for bone regeneration. We performed nanotopography on titanium plates through alkali and heat (Ti8) treatment to promote human mesenchymal stem cell (hMSC) differentiation. Next, we extracted the sEVs from the hMSC, which were cultured on the nanotopographical Ti plates for 21 days (Ti8-21-sEV). We demonstrated that Ti8-21-sEV had superior pro-osteogenesis ability in vitro and in vivo. RNA sequencing further confirmed that Ti8-21-sEV promote bone regeneration through osteogenic-related pathways, including the PI3K-AKT signaling pathway, MAPK signaling pathway, focal adhesion, and extracellular matrix-receptor interaction. Finally, we decorated the Ti8-21-sEV on a 3D printed porous polyetheretherketone scaffold. The femoral condyle defect model of rabbits was used to demonstrate that Ti8-21-sEV had the best bone ingrowth. In summary, our study demonstrated that the Ti8-21-sEV have memory function by copying the pro-osteogenesis information from the nanotopography. We expect that our study will encourage the discovery of other sEV with morphology memory for tissue regeneration.
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