生物
基因
基因沉默
大戟
异源的
异源表达
计算生物学
转录组
基因表达
遗传学
植物
重组DNA
作者
Tomasz Czechowski,Edith Forestier,Sandesh H Swamidatta,Alison D. Gilday,Amy Cording,Tony R. Larson,David Harvey,Yi Li,Zhesi He,Andrew King,Geoffrey D. Brown,Ian A. Graham
标识
DOI:10.1073/pnas.2203890119
摘要
Significance Euphorbia peplus , a member of the Euphorbia genus, is rich in jatrophane and ingenane diterpenoids. Using a metabolomics-guided transcriptomic approach to gene candidate identification, we have discovered a short-chain dehydrogenase gene involved in the production of the lathyrane jolkinol E. We have developed a virus-induced gene-silencing method in E. peplus that has allowed us to demonstrate the direct relationship between casbene and polycyclic diterpenoids and that jolkinol C acts as a key branch point intermediate in the production of ingenanes and jatrophanes. This work contributes both knowledge and tools for engineering production of bioactive diterpenoids in heterologous host systems, thus enabling their further evaluation and development.
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