脊髓损伤
脊髓
蛋白质组
椎板切除术
再生(生物学)
免疫印迹
生物
细胞生物学
蛋白质组学
医学
解剖
神经科学
生物信息学
生物化学
基因
作者
Qinxue Ding,Zhe Wu,Yong Guo,Chenyang Zhao,Yufeng Jia,Fanwen Kong,Bingyao Chen,Hongxia Wang,Shaoxiang Xiong,Haiping Que,Shuqian Jing,Shaojun Liu
出处
期刊:Proteomics
[Wiley]
日期:2006-01-01
卷期号:6 (2): 505-518
被引量:47
标识
DOI:10.1002/pmic.200500296
摘要
The inability of the CNS to regenerate in adult mammals propels us to reveal associated proteins involved in the injured CNS. In this paper, either thoracic laminectomy (as sham control) or thoracic spinal cord transection was performed on male adult rats. Five days after surgery, the whole spinal cord tissue was dissected and fractionated into water-soluble (dissolved in Tris buffer) and water-insoluble (dissolved in a solution containing chaotropes and surfactants) portions for 2-DE. Protein identification was performed by MS and further confirmed by Western blot. As a result, over 30 protein spots in the injured spinal cord were shown to be up-regulated no less than 1.5-fold. These identified proteins possibly play various roles during the injury and repair process and may be functionally categorized as several different groups, such as stress-responsive and metabolic changes, lipid and protein degeneration, neural survival and regeneration. In particular, over-expression of 11-zinc finger protein and glypican may be responsible for the inhibition of axonal growth and regeneration. Moreover, three unknown proteins with novel sequences were found to be up-regulated by spinal cord injury. Further characterization of these molecules may help us come closer to understanding the mechanisms that underlie the inability of the adult CNS to regenerate.
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