姜黄素
Zeta电位
PLGA公司
纳米颗粒
析因实验
粒径
材料科学
纳米技术
乳状液
核化学
化学
化学工程
数学
有机化学
生物化学
物理化学
统计
工程类
作者
Mohamed A. Akl,Alma Kartal-Hodzic,Timo Oksanen,Hatem R. Ismael,Mohsen M. Afouna,Marjo Yliperttula,Ahmed M. Samy,Tapani Viitala
标识
DOI:10.1016/j.jddst.2016.01.007
摘要
We have employed a 23 factorial design approach for optimizing curcumin-loaded PLGA nanoparticles with a specific interest in colon treatment. Curcumin-loaded PLGA nanoparticles were prepared by using the single emulsion solvent evaporation technique. In vitro characterizations of the curcumin-loaded nanoparticles revealed that the mean particle sizes of the nanoparticles ranged from 181.5 nm to 206.9 nm, the zeta potential values were in the range of −30.6 to −41.7 mV, the encapsulation efficiencies were between 58.1 and 83.2% and the drug release from the formulations was in the range of 34.4–62.8%. The properties of the optimized curcumin-loaded PLGA nanoparticles predicted by the 23 factorial design approach correlated very well with the experimentally determined particle size of 219.6 nm, zeta potential of −36.8 mV, encapsulation efficiency of 74.4% and a 56.2% cumulative drug release after 24 h. In vitro cellular uptake studies with HT-29 cells showed that the optimized curcumin-loaded PLGA nanoparticle exhibited a much higher cellular uptake of curcumin (i.e. 7.01 ± 0.33 μg/106 cells) than a native curcumin solution (3.74 ± 0.56 μg/106 cells). Stability studies also showed that all investigated formulations were stable at least for 2 months.
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