肿瘤坏死因子α
胸腺细胞
受体
生物
淋巴毒素
刺激
细胞生长
兴奋剂
多克隆抗体
淋巴毒素α
T细胞
内分泌学
细胞生物学
抗体
内科学
分子生物学
免疫学
医学
免疫系统
生物化学
作者
Louis A. Tartaglia,David V. Goeddel,Reynolds Cp,I S Figari,R Weber,Brian M. Fendly,Michael A. Palladino
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1993-11-01
卷期号:151 (9): 4637-4641
被引量:263
标识
DOI:10.4049/jimmunol.151.9.4637
摘要
TNF-alpha can enhance the proliferation of human thymocytes stimulated by the comitogen Con A. To determine which of the two different TNF receptors is responsible for signaling this cellular response, we investigated the proliferation of human thymocytes in response to agonistic antibodies specific for the two TNF receptor types. In contrast to previously examined TNF activities in human cells, thymocyte proliferation was stimulated in response to rabbit polyclonal antibodies directed against the 75-kDa TNF receptor (TNF-R2), but not those directed against the 55-kDa TNF receptor (TNF-R1). Lymphotoxin (TNF-beta) was also shown to stimulate human thymocyte proliferation, demonstrating that TNF-beta can initiate a biologic response that is mediated by TNF-R2. TNF-R2-mediated T-cell proliferation was not restricted to the immature T cells within the thymus, as the anti-TNF-R2 antibodies also stimulated the proliferation of peripheral T cells. As a first step toward identifying a specific agonist of TNF-R2 with therapeutic potential, 10 anti-TNF-R2 mAb were examined for potential agonist activity. Nine of these significantly stimulated human thymocyte proliferation with maximal responses ranging from twofold to significantly greater than that obtained with TNF-alpha by itself.
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