J-Aggregated Fluorescence Nanoparticles with Multichromatic Performance Enable Sensitive LFIA Platform

The application of conventional lateral flow immunoassay (LFIA) remains challenging due to its single signal readout and poor sensitivity. In this work, we developed J-aggregated fluorescence nanoparticles (JNPs) with multichromatic performance and employed them as reporters for the simultaneous and sensitive detection of T-2 and AFB1 toxins on the LFIA platform. Upon the strong confinement effect of nanoparticles, the emission region of JNPs was red-shifted from ∼545 to ∼620 nm, exhibiting a strong J-aggregation phenomenon. Subsequently, two JNPs with green emission and red emission were employed for the development of multiplexed LFIA platform (JNPs-LFIA) for on-site quantitative detection of T-2 and AFB1 toxins, with the limits of detection of 0.645 ng mL-1 and 0.0035 ng mL-1, which were 3.5-fold and 12.2-fold lower than those of AuNPs-LFIA, respectively. Overall, this work provides an efficient strategy for the design of multichromatic immunoprobes and promotes the development of reporters in biosensing application.