Inhibition of AhR disrupts intestinal epithelial barrier and induces intestinal injury by activating NF‐κB in COPD

Abstract Chronic obstructive pulmonary disease (COPD) is frequently associated with intestinal comorbidities. Damage to the intestinal barrier plays a crucial role in these disorders, leading to increased intestinal and systemic inflammation, and thereby promoting the progression of COPD. This study aims to investigate the mechanism of intestinal epithelial barrier damage, focusing on the roles of the Aryl hydrocarbon Receptor (AhR) and NF‐κB in COPD‐related intestinal damage. A COPD rat model was induced by cigarette smoke and bacterial infection, while Caco‐2/HT29 intestinal epithelial cells were treated with TNF‐α or IL‐1β to assess intestinal disorder and the underlying mechanisms of barrier damage. COPD rats exhibited significant lung function decline, pathological damage, and inflammatory response in lung tissues. Additionally, significant intestinal injury was observed, accompanied by pronounced colonic pathological damage, an enhanced inflammatory response, and intestinal barrier disruption. This was evidenced by decreased expression of apical junction proteins and elevated serum diamine oxidase levels. Pro‐inflammatory cytokines TNF‐α or IL‐1β significantly downregulated the expression of apical junction proteins in Caco‐2/HT29 cells, reduced transepithelial electrical resistance of Caco‐2 cells, and increased FD‐4 permeability. Moreover, TNF‐α or IL‐1β induction activated NF‐κB in Caco‐2/HT29 cells, with a similar activation observed in the colonic tissues of COPD rats. The NF‐κB inhibitor PDTC suppressed this activation and protected against intestinal epithelial barrier damage. Furthermore, AhR inhibition was observed both in vitro and in vivo. The AhR activator FICZ inhibited NF‐κB activation and mitigated intestinal epithelial barrier damage, whereas the AhR inhibitor CH223191 inhibited AhR and exacerbated intestinal epithelial barrier damage by facilitating NF‐κB activation. However, the NF‐κB inhibitor PDTC did not significantly affect AhR. Additionally, TNF‐α/IL‐1β inhibited the binding of AhR and p‐NF‐κB. Consequently, AhR inhibition can downregulate the expression of apical junction proteins, probably through activation of NF‐κB signaling leading to intestinal epithelial barrier damage. This study confirmed the presence of lesions in the lungs and intestines of COPD rats, as well as the associated damage to the intestinal epithelial barrier. The inhibition of AhR followed by the activation of NF‐κB has been identified as a critical mechanism underlying the injury to the intestinal epithelial barrier.